rhil 22 Search Results


rhil-22 10 ng/ml  (GenScript corporation)
90
GenScript corporation rhil-22 10 ng/ml
Rhil 22 10 Ng/Ml, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rhil-22 10 ng/ml/product/GenScript corporation
Average 90 stars, based on 1 article reviews
rhil-22 10 ng/ml - by Bioz Stars, 2026-03
90/100 stars
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rhil-22  (ACROBiosystems)
90
ACROBiosystems rhil-22
Rhil 22, supplied by ACROBiosystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rhil-22/product/ACROBiosystems
Average 90 stars, based on 1 article reviews
rhil-22 - by Bioz Stars, 2026-03
90/100 stars
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hil-22 kit  (PeproTech)
90
PeproTech hil-22 kit
Expression and secretion of <t>hIL-22</t> by L. reuteri . (A) Recombinant hIL-22 detected by ELISA in supernatants of recombinant L. reuteri (PRB484) upon induction. L. reuteri expressing pSIP411 was used as control (PRB577). (B) Induction of IL-10 production from Colo205 cells upon treatment with commercial rhIL-22 as compared to bacterially secreted IL-22. Data are mean of n = 3 with error bars representing deviation from the mean; comparisons performed with t -tests (two groups) or analysis of variance (ANOVA) (multiple groups). *** P < 0.001, **** P < 0.0001.
Hil 22 Kit, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hil-22 kit/product/PeproTech
Average 90 stars, based on 1 article reviews
hil-22 kit - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


Expression and secretion of hIL-22 by L. reuteri . (A) Recombinant hIL-22 detected by ELISA in supernatants of recombinant L. reuteri (PRB484) upon induction. L. reuteri expressing pSIP411 was used as control (PRB577). (B) Induction of IL-10 production from Colo205 cells upon treatment with commercial rhIL-22 as compared to bacterially secreted IL-22. Data are mean of n = 3 with error bars representing deviation from the mean; comparisons performed with t -tests (two groups) or analysis of variance (ANOVA) (multiple groups). *** P < 0.001, **** P < 0.0001.

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Challenges and Pitfalls in the Engineering of Human Interleukin 22 (hIL-22) Secreting Lactobacillus reuteri

doi: 10.3389/fbioe.2020.00543

Figure Lengend Snippet: Expression and secretion of hIL-22 by L. reuteri . (A) Recombinant hIL-22 detected by ELISA in supernatants of recombinant L. reuteri (PRB484) upon induction. L. reuteri expressing pSIP411 was used as control (PRB577). (B) Induction of IL-10 production from Colo205 cells upon treatment with commercial rhIL-22 as compared to bacterially secreted IL-22. Data are mean of n = 3 with error bars representing deviation from the mean; comparisons performed with t -tests (two groups) or analysis of variance (ANOVA) (multiple groups). *** P < 0.001, **** P < 0.0001.

Article Snippet: ELISA was performed using the Peprotech hIL-22 kit (Peprotech, NJ, USA), as recommended by the supplier.

Techniques: Expressing, Recombinant, Enzyme-linked Immunosorbent Assay

Bacterially secreted hIL-22 is cleaved. Western blot of induced bacterial supernatant and cell lysates. Cell lysates of PRB484 show a band at ~23 kDa, the expected size for hIL-22 fused to the signal peptide. In comparison, supernatants from induced PRB484 cultures show protein bands much smaller in size, ~4 kDa representing cleaved hIL-22.

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Challenges and Pitfalls in the Engineering of Human Interleukin 22 (hIL-22) Secreting Lactobacillus reuteri

doi: 10.3389/fbioe.2020.00543

Figure Lengend Snippet: Bacterially secreted hIL-22 is cleaved. Western blot of induced bacterial supernatant and cell lysates. Cell lysates of PRB484 show a band at ~23 kDa, the expected size for hIL-22 fused to the signal peptide. In comparison, supernatants from induced PRB484 cultures show protein bands much smaller in size, ~4 kDa representing cleaved hIL-22.

Article Snippet: ELISA was performed using the Peprotech hIL-22 kit (Peprotech, NJ, USA), as recommended by the supplier.

Techniques: Western Blot

hIL-22 is also cleaved by other Lactobacilli engineered to secrete hIL-22. (A) Western blot of hIL-22 from supernatants of L. casei harboring pLOV33 or pSIP411 showing hIL-22 secreted by L. casei is cleaved similar to L. reuteri. (B) Western blot of hIL-22 from supernatants of L. lactis harboring pLOV33 (PRB873) showing cleavage of IL-22.

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Challenges and Pitfalls in the Engineering of Human Interleukin 22 (hIL-22) Secreting Lactobacillus reuteri

doi: 10.3389/fbioe.2020.00543

Figure Lengend Snippet: hIL-22 is also cleaved by other Lactobacilli engineered to secrete hIL-22. (A) Western blot of hIL-22 from supernatants of L. casei harboring pLOV33 or pSIP411 showing hIL-22 secreted by L. casei is cleaved similar to L. reuteri. (B) Western blot of hIL-22 from supernatants of L. lactis harboring pLOV33 (PRB873) showing cleavage of IL-22.

Article Snippet: ELISA was performed using the Peprotech hIL-22 kit (Peprotech, NJ, USA), as recommended by the supplier.

Techniques: Western Blot

PRB782 shows improved processing of secreted hIL-22. (A) Western blot of bacterial supernatant from PRB782, PRB484, and PRB577 shows new strain of recombinant L. reuteri (PRB782) with production of more intact hIL-22. (B) hIL-22 ELISA showing lower concentrations of secreted hIL-22 from PRB782 as compared to PRB484. Data are mean of n = 3 with error bars representing deviation from the mean; comparisons performed with t -tests (two groups) or analysis of variance (ANOVA) (multiple groups). *** P < 0.001, **** P < 0.0001.

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Challenges and Pitfalls in the Engineering of Human Interleukin 22 (hIL-22) Secreting Lactobacillus reuteri

doi: 10.3389/fbioe.2020.00543

Figure Lengend Snippet: PRB782 shows improved processing of secreted hIL-22. (A) Western blot of bacterial supernatant from PRB782, PRB484, and PRB577 shows new strain of recombinant L. reuteri (PRB782) with production of more intact hIL-22. (B) hIL-22 ELISA showing lower concentrations of secreted hIL-22 from PRB782 as compared to PRB484. Data are mean of n = 3 with error bars representing deviation from the mean; comparisons performed with t -tests (two groups) or analysis of variance (ANOVA) (multiple groups). *** P < 0.001, **** P < 0.0001.

Article Snippet: ELISA was performed using the Peprotech hIL-22 kit (Peprotech, NJ, USA), as recommended by the supplier.

Techniques: Western Blot, Recombinant, Enzyme-linked Immunosorbent Assay

Production of hIL-22 affects the growth of L. reuteri . OD 600 measurements of PRB782, PRB484, and PRB577 before and after induction of hIL-22. Data represent the average of 3 biological replicates.

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Challenges and Pitfalls in the Engineering of Human Interleukin 22 (hIL-22) Secreting Lactobacillus reuteri

doi: 10.3389/fbioe.2020.00543

Figure Lengend Snippet: Production of hIL-22 affects the growth of L. reuteri . OD 600 measurements of PRB782, PRB484, and PRB577 before and after induction of hIL-22. Data represent the average of 3 biological replicates.

Article Snippet: ELISA was performed using the Peprotech hIL-22 kit (Peprotech, NJ, USA), as recommended by the supplier.

Techniques:

hIL-22 secreted by PRB782 induces production of the antimicrobial peptide Reg3α in human enteroids. Reg3α present in enteroids supernatants treated with PRB484, PRB782, and pRB577. Monolayer of enteroids were treated for 16 h and detection of Reg3α was done by ELISA. Data represent the average of six biological replicates. Error bars indicate the standard deviation of the mean. Comparisons performed with analysis of variance (ANOVA) (multiple groups). **** P < 0.0001.

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Challenges and Pitfalls in the Engineering of Human Interleukin 22 (hIL-22) Secreting Lactobacillus reuteri

doi: 10.3389/fbioe.2020.00543

Figure Lengend Snippet: hIL-22 secreted by PRB782 induces production of the antimicrobial peptide Reg3α in human enteroids. Reg3α present in enteroids supernatants treated with PRB484, PRB782, and pRB577. Monolayer of enteroids were treated for 16 h and detection of Reg3α was done by ELISA. Data represent the average of six biological replicates. Error bars indicate the standard deviation of the mean. Comparisons performed with analysis of variance (ANOVA) (multiple groups). **** P < 0.0001.

Article Snippet: ELISA was performed using the Peprotech hIL-22 kit (Peprotech, NJ, USA), as recommended by the supplier.

Techniques: Enzyme-linked Immunosorbent Assay, Standard Deviation